Following recent studies done by Baker (US), a new vision was gained concerning the start-up time of biological safety cabinets (BCS).
To be sure that the workspace is particle-free, 3 steps are required:
Product protection depends on how clean the workspace is. Removing pollution cannot be achieved due to air currents. You will therefore first have to clean the workspace with a cleaning agent.
After cleaning (removing contamination), disinfectants must be used. These procedures must be carried out according to GMP as well before as after the work is done:
Particles of aerosols
When cleaning and disinfecting, particles will also be released in addition to aerosols. A certain amount of time is required to remove those particles and aerosols, the amount of time depends on the amount of particles and aerosols released during cleaning. It is very likely that 90 seconds after your cleaning everything has been removed, but to be sure we advise you to keep it for at least 3 minutes after cleaning.
The research Baker conducted on the SterilGARD® stated that the number of PPM from Helium was reduced from 73,000 to 10 PPM within 60-90 seconds. This is because the number of air cycles the cabinet needs to ‘bring in the air’ back to 0. This is why volatile disinfectants need a number of air cycles to be completely out of the workspace. The complete research contains a more detailed explanation of how the investigation took place.
We, CleanAir by Baker, have adjusted the start-up time of our EF and BioVanguard BSCs to 3 minutes after the “safe” signal from the BSC.
Normally (if we follow the manufacturer and GMP-rules) the following stages are required before commissioning:
End of works:
“Continuation” work (this is where you can save time):
If you will not use the BSC for a longer period of time, we advise you to switch off the BSC, this saves energy. If you want to use the BSC again in a short time you can, to save start-up time, set the BSC to Standby / Night mode. Then you have to, in principle, just wait until the BSC indicates that it is safe to work in (you have already done cleaning / disinfecting and waiting for 3 minutes in the previous step).
Of course there may be circumstances for which this procedure does not apply. However, that is only based on a risk analysis only you can do. The estimatation of the risks of your work, can only done by you. With this information based on the research of Baker, we hope to be able to use working time more efficiently for the user.
Click on the link to look into the research done by Kara Held Ph.D. (Science Director Baker US).
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